The effect of metallic substitution on the catalytic constants for NagA might be utilized to evaluate the contributions created by these divalent cations on enzymatic action (22, 23). The connection on the worth of k
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b may be the dissociation regular in the team that have to be protonated for entire activity. The pH profiles which showed which the deprotonation of two acid ic teams as well as the protonation of one fundamental team are needed for max action have been suit to equation 5 the place K
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Mutation of H143 to an asparagine resulted from the dramatic lack of catalytic exercise and a reasonable rise in the K
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